Determination of α-Linoleic Acid,Linoleic Acid and Oleic Acid in Hemp Seed by HPLC

Objective:To establish a HPLC method for the determination of α-linoleic acid,linoleic acid and oleic acid in Hemp Seed.Method: The Kromasil 100-5 C18(4.6 mm×250 mm,5 μm) column was used with a mobile phase of acetonitrile-0.1% phosphoric acid(80∶20),the flow rate was 1 mL·min-1,the column temperature was at 30 ℃,the detection wavelength was at 203 nm.Result: The linear ranges of α-linoleic acid,linoleic acid and oleic acid were 34.625-554 mg·L-1(r=0.999 9),56.375-902 mg·L-1(r=1) and 17.125-274 mg·L-1(r=0.999 9) respectively.The average recoveries were 96.38% with RSD 0.93% for α-linoleic acid,97.79% with RSD 0.92% for linoleic acid,97.06% with RSD 1.51% for oleic acid.Conclusion: The method can be used to control the quality of α-linoleic acid,linoleic acid and oleic acid in Hemp Seed,which is simple,accurate,convenient,specific and repeatable.