Effects of insulin induced by hyperglycemia on the production of solubility thrombomodulin in human umbilical vein endothelial cells

【Objective】To determine the effects of insulin induced by hyperglycemia on the production of sTM in human umbilical vein endothelial cells(HUVEC) and the possible mechanisms.【Methods】HUVEC which were cultured in vitro were divided into glucose(Glu) group(5.5,20 and 40 mmol/L),insulin group(10 IU/mL insulin + 40 mmol/L glucose)and control group(not including glucose and insulin).The cell supernatant was collected at different time(0,6,24,48 and 72 h) and the level of sTM were detected by ELISA(enzyme linked immunosorbent assay).And at the same time,the effect of Activated partial thromboplastin time(APTT) mixed proportion with the cell supernatant from normal blood was assayed.【Results】The level of sTM of HUVEC at high concentration of Glu(40 mmol/L)was higher than that of control group and it increased with time going by after the cells exposed for 48 h(P0.05).But the level of sTM decreased after the cells exposed for 72 h(P0.05).The level of APTT at high concentration of Glu(40 mmol/L)was lower than that of control group and it decreased with time going by(P0.01)The level of sTM at high concentration of Glu with insulin was lower than the level of those cultured with Glu(P0.05).The level of APTT at high concentration of Glu with insulin increased with time going by,it was still lower than that of control group(P0.01).【Conclusion】Insulin can reduce sTM level and inhibit the damage effect of high concentration Glu on HUVEC.