Effects of High Glucose on the Production of Solubility Thrombomodulin in Human Umbilical Vein Endothelial Cells

Objective To investigate the effects of glucose on the production of sTM by human umbilical vein endothelial cells(HUVEC),and to explore the possible mechanisms of diabetic vascular complications due to damage of vein endothelial cells.Methods HUVEC cultured in vitro were divided into glucose(Glu) group(5.5,20 and 40 mmol/L),and control group(no glucose added).Cell supernatant was collected at different time points(0,6,24,48 and 72 h) and the level of sTM was detected using ELISA(enzyme linked immunosorbent assay).Meanwhile,cell supernatants were mixed with an equal volume of plasma,and the Activated Partial Thromboplastin Time(APTT) and Prothrombin Time(PT) were measured using an automatic coagulator.Results The level of sTM induced by Glu(40 mmol/L) increased over the time,and it was higher than that in the control group after 48 h exposure(P0.05).In contrast,the level of sTM decreased after cells exposure to Glu for 72 h(P0.05).The level of APTT at a high concentration of Glu(40 mmol/L),which decreased over the time,was lower than that in the control group(P0.01).There was no statistical difference in PT.There was no significant difference in sTM,APTT and PT at a glucose concentration of 5.5 and 20 mmol/L,compared to the control group.Conclusion High concentrations of Glu can damage HUVEC and lead to a high coagulation state.