The effects of propofol on the expression of HSP70 and LDH of cultured neonatal rat cardiomyocytes subjected to hypoxia/reoxygenation

Objective:To observe the effect of propofol on HSP70 expression in cultured cardiac myocytes of neonatal rats and the concentration of lactate dehydrogenase(LDH)in culture medium during reoxygenation.Methods:Original cultured cardiomyocytes of SD rats and hypoxia-reoxygenation injuried immature cardiomyocyte were established.The 36 cases of cultured cardiac myocytes were divided into 6 groups:Group I as control;Group II as hypoxia-reoxygenation(hypoxia 4 hours-reoxygenation 4 hours)control;Compared to Group II,Groups III,IV,V and VI were treated with intelipid or propofol(25μmol/L,50μmol/L and 100μmol/L)since 10min before hypoxia respectively.Immunohistochemistry and mean gray were used to estimate the effect of propofol on the expression of HSP70 in cultured rat cardiomyocytes in 4 cases of each group 4h after reoxygenation.Release of lactate dehydrogenase of the cardiomyocytes in the other 2 cases of each group were investigated and determined as injury index of cardiomyocytes.Result:Release of lactate dehydrogenase was inhibited by propofol in 3 kinds of density,and the expression of HSP70 of the cardiomyocytes was significantly inhibited by propofol in 100μmol/L(P0.05).Conclusion:Hypoxia-reoxygenation injury of the cardiomyocytes was released,and then the expression of HSP70 during reoxygenation was inhibited.