2007•Zhongguo zuzhi gongcheng yanjiu yu linchuang kangfuRequires access
Protective effects of baicalin on hypoxia/reoxygenation injury in cultured neonatal rat cardiomyocytes
FY Nanyang
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Abstract
AIM: To investigate the protective effect of baicalin on hypoxia/reoxygenation injury in cultured neonatal rat cardiomyocytes and the action mechanism. METHODS: The experiment was performed in the Department of Pharmacology, Medical College of Zhengzhou University from November 2006 to January 2007. ①Totally 20 SD rats aged 1-3 days of either sex were selected. Hypoxia/reoxygenation injured model was made with purified cultured cardiomyocytes, and the rats were divided into 5 groups, normal control group (normal cells were cultivated with only medium of the same volume), hypoxia/reoxygenation injured group, hypoxia/reoxygenation plus low concentration baicalin group (0.1 mg/L baicalin), hypoxia/reoxygenation plus middle concentration baicalin group (1 mg/L baicalin), hypoxia/reoxygenation plus high concentration baicalin group (10 mg/L baicalin). ②The releasing amounts, activities of superoxide dismutase (SOD), contents of malondialdehyde (MDA) and activities of cardiomyocytes were measured with lactic dehydrogenase (LDH), xanthine oxidase, thiobarbituric acide and tetrazolium methods, respectively. RESULTS: ①Contents of MDA and activities of LDH were significantly higher in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups (P 0.01). ②The activities of SOD were significantly lower in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups (P 0.01). ③The viabilities of cardiomyocytes were significantly lower in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups (P 0.01). CONCLUSION: Baicalin has a protective effect on cardiomyocytes with hypoxia/reoxygenation injury, which can be associated with its antioxidation and anti-free radical effects.