Protective effect of Ulinastatin on the ischemia-reperfusion injury of pancreas in rats

Objective To study the protective effect of Ulinastatin on ischemia-reperfusion injury of pancreas in rats and assess the possible mechanism.Methods Seventy-two SD rats were randomly divided into pseudoperation group,ischemia-reperfusion group and Ulinastatin group.During the pancreatic ischemia for 30 minutes and reperfusion in 0,6,12 and 24 hours,serum TNF-α and IL-1β were determined with enzyme linked immunosorbent assay(ELISA);expression of bcl-2,HSP70 in pancreas cells were studied with immunohistochemical methods and pancreas histopathology was observed,respectively.Results The expression level of TNF-α and IL-1β in ischemia-reperfusion group was obviously higher than those in pseudoperation group and Ulinastatin group(P0.05);The expression of bcl-2 in Ulinastatin group was obviously higher than those in pseudoperation group and ischemia-reperfusion group at 6 h,12 h and 24 h of reperfusion(P0.05).The expression level of HSP70 in ischemia-reperfusion group and Ulinastatin group was higher than those in pseudoperation group at 12 h and 24 h of reperfusion(P0.05).Histopathologic observation:the inflammation was alleviated in the Ulinastatin group.Conclusion Ulinastatin may reduce the expression level of TNF-α and IL-1β during the pancreatic ischemia and reperfusion,and may enhance the expression level of bcl-2.Ulinastatin has protective effects against the ischemia-reperfusion injury of the rats pancreas.