Determination of Avermectin Residue in Rice Grain and Straw with the Method of HPLC Fluorecence Detection

[Objective]The method of avermectin residue in rice grain and straw was developed through the operation of HPLC fluorecence detection.[Method] The rice grain samples were extracted with acetonitrile/ethyl acetate(1∶1,V∶V) and the rice straw samples were extracted with ethyl acetate.After derived by TFAA-NMIM system without water,the content of avermectin in the extracts was determined with HPLC-fluoresoence detector.[Results] The results suggested that in the range of adding concentration of 0.001-0.100 mg/kg,the recovery of avermectin from rice grain was 80.1%-94.5%,with the variation coefficient of 3.5%-6.2% and rice straw,77.8%-82.8%,with the variation coefficient of 1.1%-4.9%.The minimum testing concentration with the method for rice grain was 1.000 μg/kg and for rice straw,0.500 μg/kg.[Conclusion] The sensitivity and accuracy of the proposed method were fitted for the analysis of pesticide residue in rice.