Determination of flavonoid compounds in Allium Chrysanthum by high performance liquid chromatography

A method for determination of rutin and flavonols in Allium Chrysanthum by high performance liquid chromatography was established.Allium Chrysanthum powder was extracted with ethanol of 80%by ultrasonic technique.Rutin and flavonols(quercetin,kaempferol and isoharmnten)were detected by high performance liquid chromatography.The detecting condition of rutin was that the volume ratio of methanol and 0.2%phosphoric acid was 45:55 and detection wavelength was 360nm.The detection wavelength of flavonols was the same as rutin,but the volume ratio of methanol and 0.2%phosphoric acid was 40:60.The results showed that the rutin content was 0.22%,while the quercetin,kaempferol and isorhamnetin content reached 0.42%,0,0.23%respectively,and the total flavonols content was 1.63%in Allium Chrysanthum.