Research on Solid Fermentation of Ganoderma lucidum to Produce Laccase

The culture mediums and culture conditions of solid fermentation of G.lucidum to produce constitutive laccase were investigated.The optimum culture mediums were composed of substrate(the ratio of bagasse to wheat bran appropriate was 1.5∶1),0.5 % glucose,5 % ammonium sulfate,0.01 % CuSO4·5H2O,and 73 % moisture content of substrate.The best culture conditions were summed up as follows: the initial pH value was 4.0,inoculating quantity was 20 %(V∶W),and culture at 28 ℃for 8 d.The activity of the produced laccase could reach 23803 U/g dry starter eventually.