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2010•Journal of Inner Mongolia University for the NationalitiesRequires access

Advances in Heavy Metal Immunoassay

Zhigang Gao

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Abstract

Heavy metal immunoassay compared to the traditional chemical methods,immunoassays are not only fast,cheap, simple,but also high sensitivity and strong selectivity.By selecting or synthesizing bifuntional chelators,the antigen could be prepared by chelator chelating metals and covalently linking itself to protein.Finally the monoclonal antibody directed towards metal-ohelate complexes could be generated.Heavy metal immunoassay prototype assays mainly includes competitive ELISA,KinExA immunoassay,fluorescence polarization immunoassay,colloidal gold enhanced immunochromatography assay ect.

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What this paper is about

Heavy metal immunoassay compared to the traditional chemical methods,immunoassays are not only fast,cheap, simple,but also high sensitivity and strong selectivity.By selecting or synthesizing bifuntional chelators,the antigen could be prepared by chelator chelating metals and covalently linking itself to protein.Finally the monoclonal antibody directed towards metal-ohelate complexes could be generated.Heavy metal immunoassay prototype assays mainly includes competitive ELISA,KinExA immunoassay,fluorescence polarization immunoassay,colloidal gold enhanced immunochromatography assay ect.

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Available abstract

Heavy metal immunoassay compared to the traditional chemical methods,immunoassays are not only fast,cheap, simple,but also high sensitivity and strong selectivity.By selecting or synthesizing bifuntional chelators,the antigen could be prepared by chelator chelating metals and covalently linking itself to protein.Finally the monoclonal antibody directed towards metal-ohelate complexes could be generated.Heavy metal immunoassay prototype assays mainly includes competitive ELISA,KinExA immunoassay,fluorescence polarization immunoassay,colloidal gold enhanced immunochromatography assay ect.

Key concepts: Immunoassay, Chemistry, Chelation, Chromatography, Monoclonal antibody, Metal, Fluorescence polarization immunoassay, Conjugate

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