2010•Zhongguo fuyou baojianRequires access
Study on cloning vector of pMD18-T containing group A rotavirus VP7 gene
Wu Jing
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Abstract
Objective:To construct the recombinant pMD18-T vector containing group A Rotavirus VP7 gene by RT-PCR,so as to study on cloning vector of pMD18-T containing group A rotavirus VP7 gene.Methods:Through extracting the total Group A rotavirus RNA and amplifying by RT-PCR,the VP7 gene fragment was isolated and recoveried,and trsansducted into pMD18-T.After VP7 gene was extracted from the plasmid,it was detected by 1% agarose gel electrophoresis.Results:The VP7 gene was transformed into pMD18-T.After screening by blue and white plague,the recombinant DNA (vector and the VP7 gene) was selected;And the sequence analysis results were compared with those in GeneBank.Conclusion:The VP7 gene has been successfully transformed into pMD18-T vector,so as to lay a foundation for the further development of genetic engineering vaccine against rotavirus.