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2000•Carcinogenesis,Teratogenesis and MutagenesisRequires access

THE CLONING OF TRAIL cDNA AND ITS EXPRESSION IN E.COLI

Hu Yi

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Abstract

Purpose: To develop a inducible system for expression of Trail in E.coli.Methods: RT PCR and sequencing were used to clone and conform Trail gene. The production of expression was analyzed by SDS page. Results: Trail cDNA was cloned by RT PCR. After sequencing, we constructed the inducible vector for expression of Trail in E.coli. The vector was transformed with TG1. Trail was expressed by induced of changing temperature from 30℃ to 42℃. The production was conformed by SDS page. Conclusion: This study will provide new insight into physiological and pathological roles of Trail mediated cytotoxicity and its potential application to tumor immunotherapy.

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Purpose: To develop a inducible system for expression of Trail in E.coli.Methods: RT PCR and sequencing were used to clone and conform Trail gene. The production of expression was analyzed by SDS page. Results: Trail cDNA was cloned by RT PCR. After sequencing, we constructed the inducible vector for expression of Trail in E.coli. The vector was transformed with TG1. Trail was expressed by induced of changing temperature from 30℃ to 42℃. The production was conformed by SDS page. Conclusion: This study will provide new insight into physiological and pathological roles of Trail mediated cytotoxicity and its potential application to tumor immunotherapy.

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Available abstract

Purpose: To develop a inducible system for expression of Trail in E.coli.Methods: RT PCR and sequencing were used to clone and conform Trail gene. The production of expression was analyzed by SDS page. Results: Trail cDNA was cloned by RT PCR. After sequencing, we constructed the inducible vector for expression of Trail in E.coli. The vector was transformed with TG1. Trail was expressed by induced of changing temperature from 30℃ to 42℃. The production was conformed by SDS page. Conclusion: This study will provide new insight into physiological and pathological roles of Trail mediated cytotoxicity and its potential application to tumor immunotherapy.

Key concepts: Cloning (programming), Complementary DNA, clone (Java method), Vector (molecular biology), Biology, Molecular biology, Expression vector, Escherichia coli

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