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[Expression and biological activities of TRAIL fusion protein gene].

Jing Li, Yu Zheng, Hao Chen, Li Du, Cui-li Shu, Cheng Yun

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Abstract

AIM: To obtain and express the full-length of TRAIL gene in prokaryocytes. METHODS: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli. RESULTS: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis. CONCLUSION: The TRAIL-GST with biological activity has been expressed in the prokaryotic system.

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What this paper is about

AIM: To obtain and express the full-length of TRAIL gene in prokaryocytes. METHODS: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli. RESULTS: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis. CONCLUSION: The TRAIL-GST with biological activity has been expressed in the prokaryotic system.

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Available abstract

AIM: To obtain and express the full-length of TRAIL gene in prokaryocytes. METHODS: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli. RESULTS: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis. CONCLUSION: The TRAIL-GST with biological activity has been expressed in the prokaryotic system.

Key concepts: GenBank, Gene, Molecular biology, HeLa, Fusion protein, Biology, Gene expression, Sequence analysis

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