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2012•Journal of Medicine and Pharmacy of Chinese MinoritiesRequires access

DPPH Free Radical Scavenging Activities of Extracts from Orobanche pycnostachya

LI Wan-qiang

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Abstract

Objective: DPPH free radical scavenging activities of extracts from Orobanche pycnostachya were investigated.Methods: The dried and powdered plants of O.pycnostachya were extracted with 70% ethanol under reflux.The 70% ethanol extracts was extracted successively with ethyl acetate and n-butanol.Abstract:The antioxidant activities of the 70% ethanol extract(hh4) and the three fractions of ethyl acetate(hh1),n-butanol(hh2),and water(hh3) were tested using assays of the scavenging effects on DPPH.Results: The IC50 values in DPPH test with the orders,Vc(0.0718)hh1(0.0997)hh2(0.1284)hh4(0.1732)hh3(0.4538).The study showed that the ethyl acetate fraction has the strongest scavenging activity to DPPH free radicals.Conclusion:The extracts from O.pycnostachya showed some anti-oxidant,free radical scavenging activity.

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Objective: DPPH free radical scavenging activities of extracts from Orobanche pycnostachya were investigated.Methods: The dried and powdered plants of O.pycnostachya were extracted with 70% ethanol under reflux.The 70% ethanol extracts was extracted successively with ethyl acetate and n-butanol.Abstract:The antioxidant activities of the 70% ethanol extract(hh4) and the three fractions of ethyl acetate(hh1),n-butanol(hh2),and water(hh3) were tested using assays of the scavenging effects on DPPH.Results: The IC50 values in DPPH test with the orders,Vc(0.0718)hh1(0.0997)hh2(0.1284)hh4(0.1732)hh3(0.4538).The study showed that the ethyl acetate fraction has the strongest scavenging activity to DPPH free radicals.Conclusion:The extracts from O.pycnostachya showed some anti-oxidant,free radical scavenging activity.

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Available abstract

Objective: DPPH free radical scavenging activities of extracts from Orobanche pycnostachya were investigated.Methods: The dried and powdered plants of O.pycnostachya were extracted with 70% ethanol under reflux.The 70% ethanol extracts was extracted successively with ethyl acetate and n-butanol.Abstract:The antioxidant activities of the 70% ethanol extract(hh4) and the three fractions of ethyl acetate(hh1),n-butanol(hh2),and water(hh3) were tested using assays of the scavenging effects on DPPH.Results: The IC50 values in DPPH test with the orders,Vc(0.0718)hh1(0.0997)hh2(0.1284)hh4(0.1732)hh3(0.4538).The study showed that the ethyl acetate fraction has the strongest scavenging activity to DPPH free radicals.Conclusion:The extracts from O.pycnostachya showed some anti-oxidant,free radical scavenging activity.

Key concepts: DPPH, Scavenging, Chemistry, Ethyl acetate, Ethanol, Antioxidant, Radical, Butanol

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