Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana

Objective:To investigate 1,1-diphenyl-2-picrylhydrazy1(DPPH) free radical scavenging activities of extracts from Orobanche cernua var.cumana.Method:The 70% ethanol extracts from O.cernua var.cumana was extracted successively with ethyl acetate and n-butanol.The three fractions of ethyl acetate,n-butanol,and water were tested using assays of the scavenging effects on DPPH.The model of oxidative damage of human embryonic kidney cell line HEK-293 cells was induced by H2O2,MTT assay was used to evaluate the cell viability.Result:The IC50 in DPPH test were in the following orders:ethyl acetate(0.042 9 g · L-1)n-butanol(0.059 9 g · L-1)VC(0.071 8 g · L-1)water(0.330 3 g · L-1).The ethyl acetate fraction showed the strongest scavenging activity to DPPH free radicals.The three fractions of ethyl acetate,n-butanol and water could significantly improve the survival rate of injured HEK-293 cells,showing the antioxidant activities.Conclusion:These results demonstrate that the extracts from O.cernua var.cumana have some anti-oxidant and free radical scavenging activities.