Construction of shuttle expression vector in eukaryocyte plant cells

Aim:To construct the plant shuttle expression plasmids containing the target gene kringle 5. Methods: First, the cDNA was obtained from healthy human liver by use of RT PCR. Second, PCR technique was used to obtain target gene kringle 5, and its sequence was identified by DNA sequencing kit. Third, the shuttle expression plasmids were constructed by use of sub cloning technique. The target gene kringle 5 was cloned into plasmids pBI121 and pCAMBIA3301, respectively. Results: The recombinant plant expression plasmids pB1k 5 and pC33k 5 were identified to be correct, which contained plant expression promoter CaMV 35S. Conclusion: The recombinant plasmids pB1k 5 and pC33k 5 not only steadily replicate in E.coli cells, but also express in plant cells.