Applicable value of the combined detection of TP-ELISA,TRUST and TPPA in the diagnosis of syphilis

Objective: To explore the clinical significance of Treponema pallidum-enzyme linked immunosorbent assay( TP-ELISA),syphilis toluidine red untreated serum test( TRUST) and treponemapallidum particle agglutination assay( TPPA) in the diagnosis of syphilis. Methods: The syphilis in the clinical samples were screened by TP-ELISA,and the 93 positive specimens and 16 specimens with the 0. 80 to 0. 99 of S / CO value were further confirmed by TRUST and TPPA. Results: Among the 93 positive specimens,83 positive cases and 6 weakly positive were identified by TPPA,the positive coincidence rate of which was 95. 70%( 89 /93). Among93 positive syphilis cases detected by TP-ELISA,the 32 TRUST positive cases were confirmed,and which was positive by the TPPA detection too. Among the 61 weakly positive samples detected by TRUST,the 51 positive cases,6 weakly positive cases and 4 negative cases were identified by TPPA. Among 16 cases with the 0. 80 to 0. 99 of S / CO value,the 1 positive case and 1 weakly positive case were identified by TPPA. Conclusions: The syphilis screened by TP-ELISA exists false positive and false negative. The TRUST should be tested in TP-ELISA positive specimens at first,and the TPPA in negative TRUST specimen should be further detected to identify. The TPPA in specimens with the 0. 80 to 0. 99 of S / CO value should be further detected to decrease the misdiagnosis and missed diagnosis rates of syphilis.