RP-HPLC simultaneous determination of six constituents of sesquiterpenoids in oil of Curcuma wenyujin

Objective:To develop an HPLC for quantitative determination of 6 constituents of sesquiterpenoids in Curcuma wenyujin,including curdione,curcumenol,germacrone,curzerene,furanodiene and β-elemene.Methods: A Kromasil KR100-5 C_(18)(250 mm×4.6 mm,5 μm) column was adopted with mobile phase consisted of metha- nol -water (90:10) at a flow rate of 1.0 mL·min~(-1),and the detection wavelength was at 215 nm.Results:This method could simultaneously determinate of six sesquiterpenoids in Curcuma wenyujin,The linear ranges of curdi- one,curcumenol,germacrone,curzerene,furanodiene and β-elemene were 2.66-26.6,3.47-34.7,5.63- 56.3,4.22-42.2,10.2-102,7.44-74.4 μg·mL~(-1)(r≥0.9997),respectively.The average recoveries were 98.8%,96.5%,101.2%,105.4%,96.6% and 95.2% (RSD3.0%,n=5),respectively.Conclusion:The RP - HPLC is accurate,rapid and reproducible,and destroying the heat - sensitive compound of sesquiterpenoids may be avoided.It represents the components of essential oil of Curcuma wenyujin and their praeparations literally,so it can used for quality control.