Influence of bone marrow stem cell mobilization on heart function in myocardial infarction rat

ObjectiveTo investigate the influence of heart function and the possible mechanism of repairing myocardial infarction by mobilizing the bone marrow stem cell in rat.MethodsAfter injection of isoprenaline(ISO)interaperitoneally to develop acute ischemic model,rat bone marrow stem cells were mobilized by G-CSF and migrated to the site of myocardial infarction.Hearts were harvested from 24 hours to 4 weeks after administration of ISO for histopathological examination.Immunohistochemistry,HE and VG stain were used to detect infiltration of CD34 + monocytes and the regeneration of myocytes and the inhibition of fibroatrophy of ischemic myocardium.Four weeks after injection, heart function in each group was measured using a MPA-V instrument.ResultsCompared with the control group,heart function was improved in the G-CSF mobilization group.24 hours after administration of ISO,large number of infiltrative monocytes and some regenerative myocytes with positive expression of CD34 +were found in the infarct zones of the mobilization group.2 weeks after administration of ISO,there were less scar in the mobilization group.ConclusionRat bone marrow stem cells were mobilized by G-CSF and migrated to the site of myocardial infarction,and differentiated into viable cardiomyocytes or vascular endothelial.The heart function was improved remarkably by regeneration of myocytes, inhibition of fibroatrophy and protection of ischemic myocardial structure.