Influence of bone marrow stem cell mobilization on heart function in myocardial infarction rat

Objective: To investigate the influence of heart function and the possible mechanism of repairing myocardial infarction by mobilizing the bone marrow stem cell in rat. Methods: After injection of isoprenaline (ISO) interaperitoneally to develop acute ischemic model, rat bone marrow stem cells were mobilized by G-CSF and migrated to the site of myocardial infarction. Hearts were harvested after using ISO 24 and 48 hours; 4 weeks after administration of ISO for histopathological examination. Immunohistochemisty, HE and VG stain were used to detect infiltration of CD34+ monocytes and the regeneration of myocytes and the inhibition of fibroatrophy of ischemic myocardium. 4 weeks after injection heart function of each group was measured using a MPA-V instrument. Results: Compared with the control group, heart function of the G-CSF mobilization group was improved. 24 hours after administration of ISO, a large amount of infiltrative monocytes and some regenerative myocytes with positive expression of CD34+ were found in the infarct zones of the mobilization group. 4 weeks after administration of ISO, there were fewer scars in the mobilization group. Conclusions: Rat bone marrow stem cells are mobilized by G-CSF and migrated to the site of myocardial infarction, and differentiated into viable cardiomyocytes or vascular endothelial. The heart function is remarkably improved by regeneration of myocytes and inhibition of fibroatrophy and protection of ischemic myocardial structure.