Screening and Identification of Phosphate-solubilizing Bacteria from Rhizosphere Soil in Banana Orchards in Hainan and Its Influence on Growth of Banana Seedlings

In order to release invalid state of soil fixed phosphorus,23 of phosphate-solubilizing bacteria were isolated from the rhizosphere of well growing banana plants in Hainan province using National Botanical Research Institute's phosphate growth medium(NBRIP).After repeated screening and isolation,a strain named 1-7with the strongest phosphate-solubilizing capability was obtained.After 7days incubation in liquid NBRIP medium,the content of soluble phosphate reached 638.26mg/L,about 12.8% of the tricalcium phosphate was dissolved,which was 13.9times higher than the control.Through the 16 SrDNA sequence and physiological and biochemical analysis,strain 1-7was identified as Acinetobacter baumannii.Moreover,the two seasons of pot experiments showed that the plate height,stem diameter,fresh weight and biomass of banana seedlings in the treatments inoculated with the phosphate-solubilizing strain 1-7 were increased by 17.4%,10.4%,27.3%,21.1% and 13.0%,8.2%,25.2%,13.2%,respectively,compared with the control,but no significant difference by compared to chemical phosphorus treatment.