Screening and Promotion Effect of Phosphate-solubilizing Bacteria from Grapevine Rhizosphere in Weibei Semi-arid Plateau

Phosphate-solubilizing bacteria were screened and promotion effects on grapevine were studied.By using NBRIP plate method,29 strains of phosphate-solubilizing bacteria(PSB) were isolated from the grapevine rhizosphere.After seven days incubation in a liquid medium NBRIP,the soluble P in the cultures of strains JYR-12 and JYR-7 reached 673.99 mg/L and 671.64 mg/L respectively.About 13.17% of tricalcium phosphate was dissolved.In pot experiment,the growth of Cabernet sauvignon after inoculation with phosphate-solubilizing bacteria was studied.The results showed that the plant height,stem thickness,shoot dry weight and root dry weight were significantly higher in PSB treatments than in CK.After the inoculation with phosphate-solubilizing bacteria,photosynthesis indexes of grape were obviously higher than those of contrast plants.In conclusion,the inoculation of phosphate-solubilizing bacteria could significantly promote the growth of Cabernet sauvignon.Among all these inoculation methods,the best is JYR-12.