Clinical significance of high-risk HPV detection in cervical carcinoma and cervical intraepithelial neoplasia

Objective:To discuss the clinical significance of hybrid capture 2(HC-II)in the detection of high-risk HPV(hr-HPV)for the diagnosis of cervical intraepithelial neoplasia(CIN) and cervical carcinoma and association of DNA load. Methods:Totally 300 outpatients who had received HC-Ⅱ,thinprep cytologic test(TCT) and poathologic biopsy were selected from March 2009 to April 2011. Results:Among 300 cases,59 cases(19.67%)were hr-HPV infection detected by HC-Ⅱ,and the positive coincidence rate was 62.71%(31/59).TCT found that 41 cases(13.67%) were positive,and its positive coincidence rate was 73.17%(30/41).There was statistical significance in the positive coincidence rate between HC-Ⅱ and TCT(P0.05).The hr-HPV infection rate of CINⅠ-Ⅲ and SCC group were 55.56%(5/9),62.29%(9/14),78.26%(18/23) and 100%(5/5) respectively,and it was increased with the increase of the pathology classification(χ2=84.637,P=0.000).Viral load distribution results show that hr-HPV load CIN Ⅰ-cervical cancer gradually reduce.Lesions grade and viral load without correlation(r=0.0617,P0.05). Conclusion:The hr-HPV infection is an important pathogenic factors of cervical cancer,and HC-Ⅱ is high sensitivity in hr-HPVDNA.