Optimization of the Factors Affecting Genetic Transformation of Soybean Cotyledonary Node Mediated by Agrobacterium tumefacines

Three soybean genotypes were used for optimal genotype selection in transformation mediated by Agrobacterium tumefacines.The influences of A.tumefacines concentration,infection time,acetosyringone(AS) concentration and sonication treatment were studied in this research.As a result,the appropriate genotype for cotyledonary node transformation was Dongnong 50 while Heinong 41 was fit for embryo tip transformation.In the cotyledonary node transform system,the optimal infection combination was A.tumefacines concentration at OD600=0.6 with 30-40 min infection time.The perfect AS concentration was 200 μmol·L-1.The appropriate sonicate treatment was 5 s under 100 W.The study optimized soybean cotyledonary-node Agrobacterium-mediated transformation system and transformation frequency was 2.3%.