Study of Factors on the Transformation of Soybean Cotyledonary Node Cells

The main effect factors were studied by the GUS activity in Soybean cotyledonary node cells which were infected by Agrobacterium tumefaciens containing binary vector pCAMBIA1201.Main factors affecting the transformation efficiency were determined after the 1-day-old half seeds punctured 2 times with the multi-needle were incubated for 3 days on co-cultivation medium.The transformation efficiency based on transient expression of the gus gene was significantly affected by the concentration of Agrobacterium suspension,infection time and the concentration of acetosyringone(As).The optimum conditions were as followed: As 100 or 200 μmol/L,concentration of Agrobacterium OD600=0.6,infected for 30 min.