ScholarLens Back to search
2011•China BrewingRequires access

Application of response surface methodology for the optimization of acid α-amylase production by Bacillus subtilis Y-am6

Xuepeng Yang

Open publisher page 0 citations

Abstract

The response surface methodology(RSM) was used for the optimization of acid α-amylase production by Bacillus subtilis Y-am6.The optimal carbon source,optimal organic nitrogen source and optimal inorganic nitrogen source were determined as wheat bran,soybean powder and(NH4)2SO4 by single factor test.The fermentation medium was optimized by Box-Behnken design and the results were analyzed by Design Expert 7.0 software.The optimal contents of wheat bran,soybean powder and(NH4)2SO4 in medium were 8.72%,3.19% and 2.04%,respectively.When Y-am6 was cultured on the optimized medium at 37℃ and 220r/min with initial pH 5.0 for 48h,the acid α-amylase activity reached 448.7U/ml,which was 4.87 times higher than that before optimization.

About this research paper

What this paper is about

The response surface methodology(RSM) was used for the optimization of acid α-amylase production by Bacillus subtilis Y-am6.The optimal carbon source,optimal organic nitrogen source and optimal inorganic nitrogen source were determined as wheat bran,soybean powder and(NH4)2SO4 by single factor test.The fermentation medium was optimized by Box-Behnken design and the results were analyzed by Design Expert 7.0 software.The optimal contents of wheat bran,soybean powder and(NH4)2SO4 in medium were 8.72%,3.19% and 2.04%,respectively.When Y-am6 was cultured on the optimized medium at 37℃ and 220r/min with initial pH 5.0 for 48h,the acid α-amylase activity reached 448.7U/ml,which was 4.87 times higher than that before optimization.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The response surface methodology(RSM) was used for the optimization of acid α-amylase production by Bacillus subtilis Y-am6.The optimal carbon source,optimal organic nitrogen source and optimal inorganic nitrogen source were determined as wheat bran,soybean powder and(NH4)2SO4 by single factor test.The fermentation medium was optimized by Box-Behnken design and the results were analyzed by Design Expert 7.0 software.The optimal contents of wheat bran,soybean powder and(NH4)2SO4 in medium were 8.72%,3.19% and 2.04%,respectively.When Y-am6 was cultured on the optimized medium at 37℃ and 220r/min with initial pH 5.0 for 48h,the acid α-amylase activity reached 448.7U/ml,which was 4.87 times higher than that before optimization.

Key concepts: Bran, Response surface methodology, Bacillus subtilis, Amylase, Fermentation, Food science, Carbon source, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Application of response surface methodology for the optimization of acid α-amylase production by Bacillus subtilis Y-am6 — Research Paper | ScholarLens