Isolation Identification and Protective Immunity Test of Newcastle Disease Virus from Pheasant

By using SPF chicken embryo,a strain of disease virus was isolated from suspected pheasants Newcastle Disease.The results of the HA and HI tests and HIV test showed that the Hemagglutination could be inhibited by NDV positive serum,but not AIV(H9 subtypes) and EDS76.There was no death of inoculation chicken embryo treated by ND standard positive serum,which proved isolated virus was NDV.Animal regression test showed that the isolated virus injected through muscular could enable non-immune pheasant the incidence of death,and symptoms and lesions appeared consistent with natural cases.However,intramuscular injection of SPF could only make chickens infected,but no clinical symptoms.Its biological characteristics were that NHAT faster,HOT for 8min,ICPI for 1.73,IVPI for 1.45,MDT for 44 h,which indicated that the pheasant NDV isolated strains and chicken F48E9 strain showed different characteristics on biological characteristics.Guangxi chicken and pheasant were immunized by using inactivated vaccine which produced by isolated virus and Losota strain for immune effect comparison and cross-protective immunity test.The results showed that HI antibody titer generated by isolated virus and Losota inactivated immune chicken have no significant difference,and each group's movements are basically the same;losota inactivated vaccine immunized pheasant from the attack of isolated virus strain for only 80%,While the other immune groups whether to against isolated virus strain or F48E9 stain could be up to 100%.It indicated that virulence and antigenic of various NDV stains existed differences.