ANALYSIS OF PUERARIN IN TRADITIONAL CHINESE MEDICINE PO CHAI PILLS BY HPLC

The objectine is to establish a method for the determination of the active constituent puerarin, Gegen crude drug and magnolol in Po Chai Pills. A simplified HPLC procedure was developed .The effects of sample preparation, the mobile phase and detection wave length on the separation of the various components in samples were investigated. A 46mm×150mm Hypersil ODS column with a mobile phase consisting of methanol-H2O (30∶70) was used. It was detected at 252 nm and 294 nm the other mobile phase consisting of methanol-H2O (77∶23). As the results, puerarin and other components were well separated. The standard curve was linear over the concentration range of 4×10-3～21×10-2 mg/mL and the correlation coeffient was 099826; Po Chai Pills could be directly chromatographed after it was diluted by CH3OH and filtrated.