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STUDIES ON CALLUS INDUCTION AND PLANT REGENERATION OF AMORPHOPHALLUS SP

Chenggang Su

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Abstract

The corm of Amorphophallus sp. was used as explants for callus induction, bud differentiation and plant regeneration. It showed that the corms give a callus induction frequency of 100% when cultured on MS medium supplemented with BA 1.5 mg/L and NAA 1.5 mg/L. Bud differentiation rate of more than 90% was obtained when cultured on MS medium supplemented with BA 1.0～1.5 mg/L and NAA 0.2 mg/L. All adventitious buds rooted and formed whole plants in the rooting medium (MS medium supplemented with BA (0.2) mg/L and NAA 0.5 mg/L).

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What this paper is about

The corm of Amorphophallus sp. was used as explants for callus induction, bud differentiation and plant regeneration. It showed that the corms give a callus induction frequency of 100% when cultured on MS medium supplemented with BA 1.5 mg/L and NAA 1.5 mg/L. Bud differentiation rate of more than 90% was obtained when cultured on MS medium supplemented with BA 1.0～1.5 mg/L and NAA 0.2 mg/L. All adventitious buds rooted and formed whole plants in the rooting medium (MS medium supplemented with BA (0.2) mg/L and NAA 0.5 mg/L).

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Available abstract

The corm of Amorphophallus sp. was used as explants for callus induction, bud differentiation and plant regeneration. It showed that the corms give a callus induction frequency of 100% when cultured on MS medium supplemented with BA 1.5 mg/L and NAA 1.5 mg/L. Bud differentiation rate of more than 90% was obtained when cultured on MS medium supplemented with BA 1.0～1.5 mg/L and NAA 0.2 mg/L. All adventitious buds rooted and formed whole plants in the rooting medium (MS medium supplemented with BA (0.2) mg/L and NAA 0.5 mg/L).

Key concepts: Callus, Corm, Explant culture, Amorphophallus, Regeneration (biology), Botany, Murashige and Skoog medium, Chemistry

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