2010•Journal of Jiangsu UniversityRequires access

A study on transfecting green fluorescent protein gene by adenovirus vector to rat bone marrow derived mesenchymal stem cells

Liping Chen

Abstract

Objective: To investigate the biological characteristics of rat bone marrow mesenchymal stem cells(MSCs) transfected green fluorescent protein(GFP) gene by adenovirus vector.Methods: Rat bone marrow-derived MSCs were isolated by adherent culture method.Flow cytometric analyses were used to identify cells which were transfected by recombinant adenovirus-EGFP using lipofectamine2000.Results: Rat bone marrow-derived MSCs were successfully isolated and efficiently express EGFP report gene by adenovirus vectors transfection.Conclusion: Rat bone marrow-derived MSCs can be transfected by recombinant adenovirus-EGFP,which may potentially be used as a target for tissue engineering and gene therapy.

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Key concepts: Green fluorescent protein, Mesenchymal stem cell, Transfection, Bone marrow, Genetic enhancement, Viral vector, Recombinant DNA, Molecular biology