Study on the transfection of enhanced green fluorescent protein gene into murine marrow mesenchymal stem cells

Objective To found the experimental basis of MSCs tracer in vivo,with enhanced green fluorescent protein gene(EGFP)mediated by recombinant adenovirus(Ad) vector transfected murine bone marrow mesenchymal stem cells(mMSCs).Methods The mMSCs were separated and enriched by using bone marrow adherent culture and identificated in vitro,to observe the efficiency of transfection of Ad-EGFP by fluorescence microscope and flow cytometry,CCK-8 applied to assay cell proliferation activity.Results 10h after transfection MSCs began to express fluorescent,6 to 8 days reached the peak infection rate of 92.3%,28 days still expression.Proliferation of transfected cells and untransfected cells no significant difference.Conclusion Ad-EGFP can transfect mMSCs effectively and safly.