Effect of Cyclophosphamide on Micronucleus Rate in Vicia faba Root Tips

Objective:To study the optimal concentration of cyclophosphamide and evaluation standard of micronucleus tests in Vicia faba root tips.Methods:Micronucleus tests were conducted in Vicia faba root tips treated with different concentrations of cyclophosphamide.Results:Micronucleus rate in Vicia faba root tips exhibited a good reproducibility and dose-effect relationship at the concentration of cyclophosphamide below 40.0 mg/L.Mutagenesis of tested samples was observed when micronucleus index was over 1.5.Conclusion:Concentrations of cyclophosphamide between 0.8 mg/L and10.0 mg/L could be used as the positive control and micronucleus index could be used to assay mutagenicity due to its accuracy,rapidness and practical value.