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THE EFFECTS OF TEMPERATURE AND PH ON THE POLYPHENOL OXIDASE ACTIVITY OF POTATOES

Di Wang

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Abstract

The plants in vitro, calluses, tubers and buds on tuber of processing potatoes were used to test the polyphenol oxidase (PPO) activity. The PPO activities of tuber, tuber bud and callus were highest at pH 5 to 5.5, but the PPO activity of plants in vitro was not high until the pH was 8. The PPO activities varied with different varieties. The PPO activities in the plants in vitro from cvs Gannong No.1 and Atlantic were highest (16.80 0.01ΔOD/min and 37.20 0.01ΔOD/min, respectively) when the temperature was 18 to 25 ℃ ; whereas the PPO activities in plants in vitro from cvs Shepody and Snowden were higher at 5 ℃.The PPO activities in all of the tubers tested were highest at 25 ℃.

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What this paper is about

The plants in vitro, calluses, tubers and buds on tuber of processing potatoes were used to test the polyphenol oxidase (PPO) activity. The PPO activities of tuber, tuber bud and callus were highest at pH 5 to 5.5, but the PPO activity of plants in vitro was not high until the pH was 8. The PPO activities varied with different varieties. The PPO activities in the plants in vitro from cvs Gannong No.1 and Atlantic were highest (16.80 0.01ΔOD/min and 37.20 0.01ΔOD/min, respectively) when the temperature was 18 to 25 ℃ ; whereas the PPO activities in plants in vitro from cvs Shepody and Snowden were higher at 5 ℃.The PPO activities in all of the tubers tested were highest at 25 ℃.

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Available abstract

The plants in vitro, calluses, tubers and buds on tuber of processing potatoes were used to test the polyphenol oxidase (PPO) activity. The PPO activities of tuber, tuber bud and callus were highest at pH 5 to 5.5, but the PPO activity of plants in vitro was not high until the pH was 8. The PPO activities varied with different varieties. The PPO activities in the plants in vitro from cvs Gannong No.1 and Atlantic were highest (16.80 0.01ΔOD/min and 37.20 0.01ΔOD/min, respectively) when the temperature was 18 to 25 ℃ ; whereas the PPO activities in plants in vitro from cvs Shepody and Snowden were higher at 5 ℃.The PPO activities in all of the tubers tested were highest at 25 ℃.

Key concepts: Polyphenol oxidase, Callus, Catechol oxidase, In vitro, Polyphenol, Chemistry, Horticulture, Botany

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