Differential Expression of Genes Associated with Four Types of Male Sterility in Cabbage

There are four most important types of cabbage (Brassica oleracea ) male sterilities (Nigra crtoplasmic male sterility, NiCMS; Ogura cytoplasmic male sterility, OguCMS; recessive genic male sterility, Ms-cr1 and dominant genic male sterility, Ms-cd1)in China. These male sterile lines and their corresponding fertile lines were analyzed for their gene expression specificity by cDNA-AFLP. The transcript diversity reflected the different characteristics among the four types of male sterility. OguCMS had the nearest genetic distance to fertile types. The OguCMS specifically interrupted TDFs was not detected. The results suggested that there were fewer OguCMS specifically interrupted genes as they were more difficult to be detected when comparing with others. Forty six NiCMS totally, Ms-cr1 and Ms-cd1 specific TDFs were detected. Sequence analysis of representative TDFs from each group showed that NiCMS, Ms-cr1 and Ms-cd1 were related to nad4 NADH dehydrogenase, RNA recognition motif (RRM)- containing protein and calcium-transporting ATPase expression interruption respectively.