Determination of Magnolol Content in the Leaves of Magnoliae officinalis from Qinba Mountain in Different Season

[Objective] Magnolol was extracted from the leaves of Magnoliae officinalis from Qinba Mountain in April and August.The content of magnolol was measured.This provided theoretical basis for the comprehensive utilization of Magnoliae officinalis leaves.[Method] Magnolol was extracted from the leaves of Magnoliae officinalis in April and August and weighted accurately.The residue was redissolved with methanol to a 25 ml volumetric flask.The content of magnolol from the leaves of Magnoliae officinalis was determined by HPLC method.The working conditions of HPLC method were as follows: the detection wavelength of 294 nm,chromatogram column of C18(4.6 nm×150.0 nm,5 μm),the column temperature of 23 ℃,the injection volume of 15 μl,the flow rate of 1.0 ml/min,mobile phase of methanol-water(79∶21).[Result] The content of magnolol from the leaves of Magnoliae officinalis from Qinba Mountain had difference.The content of magnolol from the leaves of Magnoliae officinalis in April was 0.29‰ and the content of magnolol from the leaves of Magnoliae officinalis in August was 0.32‰.[Conclusion] The content of magnolol from the leaves of Magnoliae officinalis in August was higher than that in April.Magnolol may be extracted from the leaves of Magnoliae officinalis.