2007•Acta Laboratorium Animalis Scientia SinicaRequires access
Expression of Fusion Gene of Sendai Virus in Prokaryotic System
Zong-Yao Wang, Wang Sheng-chang, Jianhua Hu, Cheng Gao
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Abstract
Objective To express fusion gene of Sendai virus in prokaryotic system and analyze its immunogenicity.Methods PCR primers were designed according to fusion protein of Sendai virus(gi:9627219).FP(S) cDNA specific for the primary antigen form of fusion gene of Sendai virus was produced by RT-PCR and was cloned into pMD18-T vector for sequence analysis.The pMD18-T-FP(S) was digested by restriction enzymes and then subcloned into the prokaryotic expression vector pQE31.The FP(S) was expressed in E.coli M15 by IPTG induction and purified,which was confirmed by SDS-PAGE and Western blot.Results The recombinant protein FP(S) was about 26×10~3 and could react with mice-anti Sendai virus serum.Conclusion The recombinant protein FP(S) has a good immunogenicity and the results of the present study provided a good basis for detection of SeV-specific antibodies by ELISA assay.