2010•Zhongguo yufang shouyi xuebaoRequires access
Expression and antigencity analysis of Sendai virus F gene in baculovirus system.
Liandong Qu, Liangfeng Jiang, Jiasen Liu, Changde Si, Guo DongChun, Qian Jiang, Lin Huan, Han LingXia, Di Liu
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Abstract
The fusion(F) gene sequences of Sendai virus(SeV) was amplified by PCR using a pair of specific primers designed according to the sequence in GenBank.The PCR product was cloned into pFastBacTM HTa vector and transferred into DH10Bac cells.Recombinant Bacmid-F was transfected into Sf9 cells,and a fusion protein about 65 ku was expressed and identified by SDS-PAGE analysis.The antigenicity of the recombinant F protein was confirmed by western blot and ELISA.