H_2O_2、LPS and TNF-αinduced injury of human umbilical vein endothelial cells

Objective To investigate the method of establishing human umbilical vein endothelial cells(HUVECs)injury model,and to provide experimental basis for the research of vascular endothelial cells in vitro.Methods HUVECs were separated and cultured,the hydrogen peroxide(H2O2),lip polysaccharide(LPS)and tumor necrosis factor-alpha(TNF-α)with different concentrations were used to induce the cell damage.MTT assay was used to determine the vitality of cells(OD value)at different time interval.Results OD value of H2O2 damage groups was significantly lower than that of normal control group(P0.01).No significant differences were found among H2O2 damage groups(P0.05).No significant differences were found between 0.1μg/mL LPS damage group and normal control group(P0.05).OD value of the other LPS damage groups was significantly lower than that of normal control group(P0.05).The effect of LPS on HUVECs was time and concentration dependent within a certain range.OD value of TNF-αdamage groups was significantly lower than that of normal control group(P0.01).The effect of TNF-αon HUVECs was time and concentration dependent within a certain range.Conclusion The injury model of HUVECs could be induced by H2O2,LPS and TNF-αin vitro.