2000•Reproduction and contraceptionRequires access
Evaluation of the Clinical Use of Intracytoplasmic Sperm Injection after Fertilization Failure in vitro
Zi‐Jiang Chen
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Abstract
Objective To evaluate the clinical use of intracytoplasmic sperm injection when fertilization cycles in vitro failed. Methods Reinsemination by using of intracytoplasmic sperm injection was carried out 24 h after oocytes retrieval in the failed fertilization cycles in vitro. Results A total of 127 one-day-old Mu oocytes from 14 failed fertilization cycles in vitro were subjected to intracyto- plasmic sperm injection. Among them, 105 were survived(82. 7%), 15 polyploidic (11. 8%), and 71 (55. 9%) fertilized normally, of which, 61(85.9%) cleaved. Thirty-two embryos were trans- ferred to 13 patients. One patient (7.7%) conceived after ET. Conclusions Intracytoplasmic sperm injection can be used when fertilization cycles in vitro failed for either diagnostic or thera- peutic purpose, but the pregnancy rates seems not high.