Detect Parallel the Point Mutation Pattern of p53 Gene in Hepatic Cellular Cancer by the Technique of Oligonucleotide Chips

Objective To detect parallelly the pattern of p53 gene point mutation from the paraffin specimen of hepatic cellular cancer(HCC).Methods Oligonucleotide chip which spoted 7 altofrequency mutation sites according to international data base of p53 gene mutation was designed,and p53 point mutation was detected with the technique of oligonucleotide chips.Results The frequency of p53 point mutation from the groups of HCC high and low occurrence were 64 3% and 14 3% respectively, P 0 05,and the main mutational form was 249ser (77 8%,among all mutation).Conclusions The point mutational hotspot of p53 gene from HCC high prevalent occurs at coding region 249(G T).Oligonucleotide chips can be applied to detect gene point mutation parallelly,high efficiently and at multipoint. China J Cancer Prev Treat,2003,10(5):452-454