Analysis of point mutation of p53 gene codon 249 in hepatocellular carcinoma of non-prevalent areas in Anhui, China

Objective： The current study was designed to investigate the point mutation of p53 gene codon 249 in hepatocellular carcinoma(HCC) of non- prevalent areas in Auhui, China. Methods： The PCR- RFLP was applied to analyze the rate of point mutation of p53 gene codon 249. Results： In this 38 cases of hepatocellular carcinoma, no homozygous deletion of p53 exon7 was detected and point mutation of codon 249 was found in 4 cases with a mutation rate of 10.53％ (4/38). Conclusion： Our data suggest that in the non- prevalent areas in Anhui, China, the incidence of point mutation of p53 codon 249 is not high in hepatocellular carcinoma, and the point mutation of p53 exon7 may not play a major role in the carcinogenesis of hepatoma.