Study on Subchronic Toxicity of Bensultap to Rats

Objectives To study the subchronic toxicity of bensultap to rats and to provide the toxicity mechanism,threshold dose and its NOAEL(no observed adverse effect level). Methods The rats were gavaged with bensultap at the dose of 135.0,67.50,22.50 mg/kg(female rats) and 157.5,78.75,26.25 mg/kg(male rats) once a day for 90 days.General conditions were observed,weight gains were checked,Blood routine and biochemical indicators were tested.Tissue samples were examined. Results 8 weeks after intoxication,the rats in high dose group showed hair fluffy,less activity,weight loss.Aspartate aminotransferase(AST) increased.Liver and kidney index(male rats) also increased.Histopathologic examination revealed that hepatocytes appeared cloudy swelling and hyalomitome appeared porous,portal tract had inflammatory cells infiltration.In medium dose group,AST increased significantly,kidney index increased in male rats,and hepatocytes appeared the same as the high dose group.No significant difference was found in low dose group. Conclusion Bensultap has toxic effect on liver and kidney.NOAEL is 22.50 mg/kg(female rats) and 25.25 mg/kg(male rats).