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2014•Laboratory animal scienceRequires access

Cryopreservation of Mongolian gerbil 2-cell Embryos by Vitrification based on EG with Two Steps

Guo Hong-gan

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Abstract

Objective Using the thyleneglycol( EG)-based solutions with two steps to freeze the 2-cell embryos of Mongolian gerbil( Meriones unguiculatus) and to appraise the effect of embryo cryopreservation. Method The embryos were exposed serially to EFS20 for 3 minutes and EFS40 for 1 minute. The embryo trealed were immersed into liquid nitrogen. Result The embryo recovery rate was 86. 73% and after freezing-thawing it survival rate was82. 94%. And about 30% of the freezing-thawing embryos developed to blastocyst in vitro. Conclusion This results demonstrates that Mongolian gerbil embryos can be safely cryopreserved using vitrification solutions based on EG with two steps.

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What this paper is about

Objective Using the thyleneglycol( EG)-based solutions with two steps to freeze the 2-cell embryos of Mongolian gerbil( Meriones unguiculatus) and to appraise the effect of embryo cryopreservation. Method The embryos were exposed serially to EFS20 for 3 minutes and EFS40 for 1 minute. The embryo trealed were immersed into liquid nitrogen. Result The embryo recovery rate was 86. 73% and after freezing-thawing it survival rate was82. 94%. And about 30% of the freezing-thawing embryos developed to blastocyst in vitro. Conclusion This results demonstrates that Mongolian gerbil embryos can be safely cryopreserved using vitrification solutions based on EG with two steps.

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Available abstract

Objective Using the thyleneglycol( EG)-based solutions with two steps to freeze the 2-cell embryos of Mongolian gerbil( Meriones unguiculatus) and to appraise the effect of embryo cryopreservation. Method The embryos were exposed serially to EFS20 for 3 minutes and EFS40 for 1 minute. The embryo trealed were immersed into liquid nitrogen. Result The embryo recovery rate was 86. 73% and after freezing-thawing it survival rate was82. 94%. And about 30% of the freezing-thawing embryos developed to blastocyst in vitro. Conclusion This results demonstrates that Mongolian gerbil embryos can be safely cryopreserved using vitrification solutions based on EG with two steps.

Key concepts: Vitrification, Cryopreservation, Gerbil, Embryo, Blastocyst, Andrology, Embryo cryopreservation, Biology

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Cryopreservation of Mongolian gerbil 2-cell Embryos by Vitrification based on EG with Two Steps — Research Paper | ScholarLens