Apoptosis and expressions of Bcl-2/Bax and Fas/FasL in decidua tissue of spontaneous abortion mouse models

Objective To study the pathogenetic mechanism of spontaneous abortion by determining apoptosis and expressions of Bcl-2 and Bax in deciduas tissue of spontaneous abortion mouse models and normal pregnancy mouse models. Methods The abortion-prone CBAXDBA/2 matings were established as the model of spontaneous abortion and the normal CBAXBALB/c matings were used as the model of normal pregnancy. SABC method was used to detect the expressions of Bcl-2 /Bax and Fas/FasL in decidua tissue of spontaneous abortion and normal pregnancy model at day 13 of gestation. Medical color-image measure system for immune-histochemistry was used for semiquantitative analysis of the expressions. Apoptosis was detected by using DNA in situ terminal deoxynuclotidy1 transferas-mediated dUTP-biotin nick ending labeling (TUNEL). Results In the decidua tissue of the spontaneous abortion models, the Bcl-2 expression was lower but the expressions of Bax and FasL were higher compared with normal models (P0.01). The expression of Fas was no significant difference in the two models (P0.05). In spontaneous abortion model, apoptotic index (AI) of decidua tissue was significantly higher than that of normal pregnancy model (P0.01). Conclusion Breakage of the regulation of decidual tissue apoptosis may directly or indirectly results in spontaneous abortion during early gestation. The pathways of Bcl-2/Bax and Fas/FasL systems may be one of the mechanisms of deciduas apoptosis in early gestation.