Effection of lentiviral-delivered PLCγ1 siRNA on human colorectal carcinomas cell lines

Objective: To preare recombinant lentivirus stably suppressing PLCγ1 in human colorectal carcinomas Lo-Vo cells, so as to establish LoVo cell lines deficient in PLC1 and to investigate the role of this gene. Methods: Recombinant lentivirus produceing PLC-γ1 siRNA were prepared. After LoVo cells were transduced with lentivirust, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLCγ1 was examined by Western-blot and RT-PCR analysis. The effect of the lentivirus on the cell proliferation and cell adhesion was analyzed by XTT method and ctll adhesion assay, respectively. Results: PLCγ1 siRNA knocked down PLCγ1 expression in LoVo cells obviously. The silenced efficiency of siRNA transducted by recombinant lentivirus was very high. Adhesion of human colorectal carcinomas LoVo cell Lines was significantly decreased, while proliferation was not affected. Conclusion: Our research confirm that PLCγ1 plays an important role in cell adhesion of colorectal carcinomas, and provides experimental evidences for targeting PLCγ1 in gene therapy against cancer.