Effects of Shenfu injection pretreatment on intestinal epithelium of endotoxemia rats

Objective:To explore the effects of Shenfu injection pretreatment on intestinal epithelium of endotoxemia rats.Methods: Forty-eight adult male rats were randomly divided into endotoxemia model group(group M,n=24)and Shenfu injection pretreatment group(group S,n=24).Rats in group M and group S were further divided into 2 h,6 h,12 h subgroups and each subgroup had eight rats.A single caudal venous injection of lipopolysaccharide(10 mg/kg)was used to induce endotoxemia model.Thirty minutes before endotoxemia model was induced,rats in group M and group S respectively intraperitoneally received saline(5 ml/kg)and Shenfu injection(5 ml/kg)and samples were respectively obtained at each time point.Another eight rats did not do any treatment were taken as control group(group C,n=8)and samples was collected directly.Blood was collected through abdominal aorta to measure intestinal fatty acid binding protein(i-FABP)and diamine oxidase(DAO)in serum by ELISA.Distal ileum was collected;levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in intestinal mucosa were detected by ELISA;morphological changes were observed by HE for staining and pathological scores were evaluated according to Chiu's score.Results:I-FABP and DAO levels in blood plasma,TNF-α and IL-6 levels in intestinal mucosa and Chiu's scores in group M were all significantly increased at each time point compared with those in group C(P0.05).At 2 h after endotoxemia model establishment,i-FABP,DAO,TNF-α and IL-6 levels as well as Chiu's scores in group S were lower than those in group M(P0.05),meanwhile,TNF-α and IL-6 levels in group S were lower than those in group M(P0.05).Conclusions:Shenfu injection pretreatment on endotoxemia rats could attenuate inflammatory response,decrease serum i-FABP and DAO levels,lower pathological scores and alleviate the injury of intestinal epithelium at early stage.