Study on the Pollination Methods and Embryo Rescue Techniques of the Crosses among Lilium Oriental Hybrids

The pollination methods and embryo rescue techniques of the crosses among LiliumOriental Hybrids were studied.The results showed as follows:In ‘Justina'בShela'combination,the pollen tubes were observed to grow normally downward in the pistils and target normally to the ovules when the normal stigma pollination method was used,but grow at random on the stylar cut and in the ovary while the cutstyle pollination method was used.The longer style length was left,the more pollen tubes targeting to ovules were observed and the more plump seeds were produced.The highest numbers of plump seeds were produced with the normal stigma pollination method.It was investigated that of the 56cross combinations among Oriental Hybrids,one combination generated no plump ovule,three combinations generated fewer than 10plump ovules and the rest 52combinations generated lots of plump ovules.This indicated that the normal stigma pollination method could be employed as a general pollination method in the crosses of Oriental Hybrids.Different embryo rescue techniques were employed The faster germination and the higher germination rate were observed by using the cut-ovule inoculation than the normal ovule inoculation on about 70days after pollination.The technique of cut-ovule inoculation in about 70days after pollination wasalso more efficient than that of embryo denuding inoculation on 40days after pollination,which could be used as a general embryo rescue method in the crosses of Oriental Hybrids.