Pollination Methods and Embryo Rescue Techniques of Hybridization between Lilium Oriental Hybrids

The pollination methods and embryo rescue techniques of hybridization between Lilium Oriental hybrids were studied in this paper. The results showed that:( 1) in the combination of‘Justina’בShela’,pollen tubes were observed to grow downward in styles and target to ovules in ovaries when normal stigma pollination method was used; pollen tubes were found to grow directionless to a different degree at stylar cuts and in ovaries when cut-style pollination method was used; the longer the styles were left,the more pollen tubes toward to ovules were observed and more plump seeds were produced; the maximum plump seeds were obtained via normal stigma pollination.( 2) Hybridization experiments were performed for the 56 combinations of Lilium Oriental hybrids with normal stigma pollination,and one combination generated no plump ovule and three combinations generated fewer than 10 plump ovules while the other 52 combinations generated plenty of plump ovules,which preliminarily indicated that normal stigma pollination method could be used as an universal pollination technique for hybridization between Lilium Oriental hybrids. The experiment results of different embryo rescue techniques showed that using cut-ovule about 70 d after pollination had a faster germination speed and a higher germination rate of hybrid embryo than normal ovule inoculation,and embryo rescue efficiency of the former method was obviously higher than embryo isolated inoculation on 40 d after pollination. The research suggested that cut-ovule inoculation about 70 d after pollination could be regarded as an universal embryo rescue technique for hybridization between Lilium Oriental hybrids.