Study on all- time double- wavelength fusion fingerprints of water extract of Cinnamomi Ramulus and quantitative analysis of six components by HPLC

Objective: To establish the double- wavelength fusion high performance liquid chromatographic fingerprints of water extract of Cinnamomi Ramulus and to determine the contents of protocatechuic acid,protocatechualdehyde,cumarin,cinnamic alcohol,cinnamic acid and cinnamaldehyde in the extract,so as to provide a scientific basis for the quality control of Cinnamomi Ramulus water extract. Methods: The column was Waters Symmetry RP C18( 4.6 mm ×250 mm,5 μm); The mobile phase,consisting of solvent A( acetonitrile) and solvent B( 0. 1% aqueous phosphoric acid),was programmed as a linear gradient( 0- 10 min,3% A→6% A; 10- 20 min,6% A→10% A; 20- 35 min,10% A→18% A; 35- 50 min,18% A→25% A; 50- 80 min,25% A→30% A; 80- 90 min,30% A→40% A); The flow rate was 1. 0 mL·min- 1; The absorbance was monitored at the wavelengths of 254 and275 nm; The column temperature was 30 ℃. Matlab 7. 1 was adopted for all- time double- wavelength fusion for the data in CSV format. Results: The calibration curves of protocatechuic acid,protocatechualdehyde,coumarin,cinnamic alcohol,cinnamic acid and cinnamaldehyde were all in good linearity over the ranges of 0. 37- 14. 9,0. 05- 2. 05,0. 58- 23. 37,0. 39- 15. 66,0. 67- 26. 85,2. 48- 99. 23 μg·mL- 1( r 0. 9995),respectively,and the average recoveries( n = 6) ranged from 97. 8% to 102. 0%,with RSD 3. 0%. The results of double-wavelength fingerprint chromatograms showed all- around information of the fingerprints at 254 and 275 nm. The fingerprints containing 12 co- possessing peaks were obtained when choosing cinnamaldehyde peak as the reference peak. The similarity among 20 batches of Cinnamomi Ramulus was over 0. 90; The information of the fusion fingerprints was more abundant. The contents of the six compounds were different in the 20 batches of Cinnamomi Ramulus. Conclusion: The systematic quantified fingerprint method based on the technique of double- wavelength fusion fingerprint and the determination of the six constituents can be used for the quality control of the water extract of Cinnamomi Ramulus.