Molecular Cloning and Sequences Analysis of Flavanone 3-hydroxylase Gene from Fagopyrum tataricum

For the purpose of enhancing the key enzymes' activity to increase flavonoids synthesis in the biosynthetic pathway of flavonoids,one of the most important key enzymes flavanone 3-hydroxylase (F3H) gene was cloned from Fagopyrum tataricum.Based on cDNA sequence conserved domain of F3H,a pair of primers were designed and used to amplify a fragment of FtF3H gene (GenBank accession:FJ456858) using RT-PCR and RACE technique.A 1 369 bp full-length cDNA sequence was obtained.Analysis of F3H cDNA indicated that it encoded a peptide containing 367 amino acids.The sequence identity to other species was all about 80%.The predicted secondary structure demonstrated that random coil were the most important structural conformation.However,alpha helix and beta pleated sheet distributed in the whole protein.The predicted tertiary structure demonstrated that FtF3H has 2-oxoglutarate iron combination sites.